Russell's Blog

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A sequencer of our own

Posted by Russell on January 27, 2011 at 4:12 p.m.
We just finished running our new GS Jr. gene sequencer for the first time. It produced 115,698 shotgun reads of our E. coli. Here is the read length histogram :

And the GC content histogram :

This was our first time going through the shotgun library protocol, which is pretty involved. For example, we're going to have to be more careful next time when we load the picotiter plate. We got a few bubbles trapped in there. It's kind of funny how obvious the bubbles are in the raw florescence images (this is an A, around cycle 200) :

I've uploaded the FASTA file and the qual file, in case you want to try to assemble your own E. coli genome.